Shipping and storage The product is shipped as a lyophilized powder at room temperature. Upon receipt, it should be stored at -20°C. Protect from moisture.
Solubility Soluble in water. For long-term storage in solution, we recommend to prepare a stock solution by dissolving the product in ddH2O at a concentration X100-1000 of final working solution. Divide the solution into small aliquots and store at -20°C. Upon use, thaw the relevant vial intended for use and dilute in your desired working buffer. Centrifuge all product preparations before use (10000 x g, 5 min). Repeat freeze-thawing might result in loss of activity. [It is recommended to prepare working solutions fresh before use].
Storage of solutions The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the toxin in working solutions for longer than a few days. Avoid multiple freeze- thaw cycles.
Our bioassay
Alomone Labs ω-Grammotoxin SIA inhibits CaV2.2 channel currents heterologously expressed in Xenopus oocytes.
A. Representative time course of ω-Grammotoxin SIA (#STG-450) inhibition of CaV2.2(α1B + α2δ1 + β1) channels current. Membrane potential was held at -100 mV, current was elicited by a 100 ms voltage ramp to +60 mV every 10 sec, and inhibited by 500 nM ω-Grammotoxin SIA(green). B. Superimposed traces of CaV2.2channel currents in the absence (control) and presence (green) of 500 nM ω-Grammotoxin SIA(taken from the recording in A).
ω-Grammotoxin SIA is a 36 amino acid peptidyl toxin originally isolated from the venom of the South American Tarantula spider, Grammostola spatulata. ω-Grammotoxin SIA potently inhibits both CaV2.1 (P-type) and CaV2.2 (N-type) Ca2+ channels by altering the voltage-dependence of channel gating. ω-Grammotoxin SIA caused a concentration-dependent and virtually complete inhibition of K+-evoked influx of 45Ca2+ into either rat or chick brain synaptosomes.1 ω-Grammotoxin SIA at 1 µM, a maximally effective concentration, blocked 52% of IBa in cultured rat hippocampal neurons.2 A concentration of >50 nM toxin completely inhibited Ca2+ currents in Purkinje neurons (predominantly, P-type channels) and in sympathetic neurons (predominantly, N-type channels).3
Target P-type and N-type Ca2+ channels
Peptide Content: 100%
Lyophilized Powder
Omega-grammotoxin SIA (#STG-450) is a highly pure, synthetic, and biologically active peptide toxin.
Alomone Labs ω-Grammotoxin SIA inhibits CaV2.2 channel currents heterologously expressed in Xenopus oocytes.A. Representative time course of ω-Grammotoxin SIA (#STG-450) inhibition of CaV2.2 (α1B + α2δ1 + β1) channels current. Membrane potential was held at -100 mV, current was elicited by a 100 ms voltage ramp to +60 mV every 10 sec, and inhibited by 500 nM ω-Grammotoxin SIA (green).
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