Overview
Type: Synthetic peptide
Form: Lyophilized Powder
Opsin 3 (extracellular) Blocking Peptide (#BLP-OR023) is the original antigen used for immunization during Anti-Opsin 3 (extracellular) Antibody (#AOR-023) generation. The blocking peptide binds and ‘blocks’ Anti-Opsin 3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Anti-Opsin 3 (extracellular) Antibody (#AOR-023), (1:200).
3-4. Anti-Opsin 3 (extracellular) Antibody, preincubated with Opsin 3 (extracellular) Blocking Peptide (BLP-OR023).
Western blot analysis of rat eye lysate:1. Anti-Opsin 3 (extracellular) Antibody (#AOR-023), (1:200).
2. Anti-Opsin 3 (extracellular) Antibody, preincubated with Opsin 3 (extracellular) Blocking Peptide (BLP-OR023).
Western blot analysis of Malme-3 skin fibroblast cell line lysates (lanes 1 and 5), human THP-1 monocytic leukemia cell line lysate (lanes 2 and 6), human Jurkat T-cell leukemia cell line lysate (lanes 3 and 7) and human ARPE-19 retinal pigmental epithelia cell line lysate (lanes 4 and 8):1-4. Anti-Opsin 3 (extracellular) Antibody (#AOR-023), (1:200).
5-8. Anti-Opsin 3 (extracellular) Antibody, preincubated with Opsin 3 (extracellular) Blocking Peptide (BLP-OR023).
Expression of Opsin 3 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Opsin 3 (extracellular) Antibody (#AOR-023), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the CA3 region, showed Opsin 3 immunoreactivity (green), in the pyramidal layer (P, left pointing arrows) and in the mossy fiber terminal zone (MFT, right pointing arrows). B. Pre-incubation of the antibody with Opsin 3 (extracellular) Blocking Peptide (BLP-OR023), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of Opsin 3 in rat optic tract.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Opsin 3 (extracellular) Antibody (#AOR-023), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Opsin 3 immunoreactivity (green), appears in the optic tract (OT, arrows). B. Pre-incubation of the antibody with Opsin 3 (extracellular) Blocking Peptide (BLP-OR023), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)DIHGLG(S)TVDWRSKD, corresponding to amino acid residues 180 - 194 of mouse Opsin 3 (Accession Q9WUK7).
Accession (Uniprot) Number Q9WUK7
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
