Overview
Type: Synthetic peptide
Form: Lyophilized powder
Orai1 (extracellular) Blocking Peptide (#BLP-CC062) is the original antigen used for immunization during Anti-Orai1 (extracellular) Antibody (#ACC-062) generation. The blocking peptide binds and ‘blocks’ Anti-Orai1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat spleen (lanes 1 and4), rat pancreas (lanes 2 and 5) and mouse B-cell lymphoma (WEHI) (lanes 3 and 6) lysates:1-3. Anti-Orai1 (extracellular) Antibody (#ACC-062), (1:200).
4-6. Anti-Orai1 (extracellular) Antibody, preincubated with Orai1 (extracellular) Blocking Peptide (#BLP-CC062).
Expression of Orai1 in rat pancreasImmunohistochemical staining of rat pancreas paraffin embedded sections using Anti-Orai1 (extracellular) Antibody (#ACC-062), (1:50). Orai1 (brown) is expressed in acinar cells. Hematoxilin is used as the counterstain.
Expression of Orai1 in rat parietal cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Orai1 (extracellular) Antibody (#ACC-062), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. A. Orai1 immunoreactivity (green) appears in neuronal profiles (arrows). B. Pre-incubation of the antibody with Orai1 (extracellular) Blocking Peptide (BLP-CC062), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of Orai1 in rat hypothalamus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Orai1 (extracellular) Antibody (#ACC-062), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. A. Orai1 immunoreactivity (green) appears in neuronal profiles (arrows). B. Pre-incubation of the antibody with Orai1 (extracellular) Blocking Peptide (BLP-CC062), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = third cerebral ventricle.
Properties
Sequence
- (C)KFLPLKRQAGQPS, corresponding to amino acid residues 200-212 of mouse Orai1 (Accession Q8BWG9).
Accession (Uniprot) Number Q8BWG9
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
