Overview
Type: Synthetic peptide
Form: Lyophilized powder
Orai2 Blocking Peptide (#BLP-CC061) is the original antigen used for immunization during Anti-Orai2 Antibody (#ACC-061) generation. The blocking peptide binds and ‘blocks’ Anti-Orai2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human HL-60 promyelocytic leukemia (lanes 1 and 3) and human Jurkat acute T cell leukemia (lanes 2 and 4) cell lysates:1,2. Anti-Orai2 Antibody (#ACC-061), (1:200).
3,4. Anti-Orai2 Antibody, preincubated with Orai2 Blocking Peptide (#BLP-CC061).
Expression of Orai2 in rat lungImmunohistochemical staining of paraffin-embedded rat lung sections using Anti-Orai2 Antibody (#ACC-061), (1:100). Strong and specific staining is evident in bronchiolar epithelium (blue) and alveoli walls (red). Hematoxilin is used as the counterstain.
Expression of Orai2 in mouse cerebellum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Orai2 Antibody (#ACC-061), (1:300), followed by goat anti-rabbit-Alexa-488. A. Orai2 immunoreactivity (green), appears in Purkinje cells (vertical arrows) and in their dendritic tree (horizontal arrows). B. Pre-incubation of the antibody with Orai2 Blocking Peptide (#BLP-CC061), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- CPEPGHKGMDYRDWVRR, corresponding to amino acid residues 16-32 of mouse Orai2 (Accession Q8BH10).
Accession (Uniprot) Number Q8BH10
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
