Overview
Type: Synthetic peptide
Form: Lyophilized powder
Orexin Receptor 2 Blocking Peptide (#BLP-OR002) is the original antigen used for immunization during Anti-Orexin Receptor 2 Antibody (#AOR-002) generation. The blocking peptide binds and ‘blocks’ Anti-Orexin Receptor 2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human Colo-205 (lanes 1 and 3) and HT-29 (lanes 2 and 4) colon cancer cell lysates:1,2. Anti-Orexin Receptor 2 Antibody (#AOR-002), (1:200).
3,4. Anti-Orexin Receptor 2 Antibody, preincubated with Orexin Receptor 2 Blocking Peptide (#BLP-OR002).
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-Orexin Receptor 2 Antibody (#AOR-002), (1:400).
3,4. Anti-Orexin Receptor 2 Antibody, preincubated with Orexin Receptor 2 Blocking Peptide (#BLP-OR002).
Expression of OX2R in rat brainImmunohistochemical staining of rat brain using Anti-Orexin Receptor 2 Antibody (#AOR-002), (1:50), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (1:500), (red). A. In the cerebellum, staining is present in cell bodies of both Golgi type I (Purkinje cells – white arrows) and Golgi type II (green arrows) neurons. Staining is also present in fibers in the molecular layer (ML). B. In the parietal cortex, staining is evident in neural cell bodies. C. In the hippocampus, staining is present in pyramidal cells in the CA1 layer. Note that both cell bodies and prolongations are stained. Hoechst 33342 is used as the counterstain (blue).
Properties
Sequence
- (C)DRLARGRTSTESRKS, corresponding to amino acid residues 391-405 of rat OX2R (Accession P56719).
Accession (Uniprot) Number P56719
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
