Cat #: BLP-OR002
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- (C)DRLARGRTSTESRKS, corresponding to amino acid residues 391-405 of rat OX2R (Accession P56719).
Accession (Uniprot) Number P56719
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of human Colo-205 (lanes 1 and 3) and HT-29 (lanes 2 and 4) colon cancer cell lysates:1,2. Anti-Orexin Receptor 2 Antibody (#AOR-002), (1:200).
3,4. Anti-Orexin Receptor 2 Antibody, preincubated with Orexin Receptor 2 Blocking Peptide (#BLP-OR002). - Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-Orexin Receptor 2 Antibody (#AOR-002), (1:400).
3,4. Anti-Orexin Receptor 2 Antibody, preincubated with Orexin Receptor 2 Blocking Peptide (#BLP-OR002). - Rat small intestine sections (paraffin embedded), (1:50).
Last update: 02/01/2024