Overview
Type: Synthetic peptide
Form: Lyophilized Powder
OTOP1 Blocking Peptide (#BLP-HC005) is the original antigen used for immunization during Anti-OTOP1 Antibody (#AHC-005) generation. The blocking peptide binds and ‘blocks’ Anti-OTOP1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney membranes (lanes 1 and 5), mouse heart lysate (lanes 2 and 6), mouse brain lysate (lanes 3 and 7) and rat tongue lysate (lanes 4 and 8):1-4. Anti-OTOP1 Antibody (#AHC-005), (1:200).
5-8. Anti-OTOP1 Antibody, preincubated with OTOP1 Blocking Peptide (#BLP-HC005).
Expression of Otopetrin-1 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Otopetrin-1 Antibody (#AHC-005), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the mouse hippocampal CA1 region, showed Otopetrin-1 immunoreactivity (green) in astrocytic profiles (arrows). B. Pre-incubation of the antibody with Otopetrin-1 Blocking Peptide (BLP-HC005), suppressed staining. Cell nuclei are stained with DAPI (blue). P= pyramidal layer, SR = stratum radiatum
Expression of Otopetrin-1 in rat cerebellum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Otopetrin-1 Antibody (#AHC-005), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Otopetrin-1 immunoreactivity (green) appeared in Bergmann glial profiles (arrows). B. Pre-incubation of the antibody with Otopetrin-1 Blocking Peptide (BLP-HC005), suppressed staining. Cell nuclei are stained with DAPI (blue). MOL = molecular layer. P= purkinje layer.
Properties
Sequence
- Peptide (C)EKSLDESKNPARKLD, corresponding to amino acid residues 374 - 388 of mouse Otopetrin-1 (Accession Q80VM9).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
