Overview
Type: Synthetic peptide
Form: Lyophilized powder
Oxytocin Receptor Blocking Peptide (#BLP-VR013) is the original antigen used for immunization during Anti-Oxytocin Receptor Antibody (#AVR-013) generation. The blocking peptide binds and ‘blocks’ Anti-Oxytocin Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membrane (1:200), rat testis and mouse testis lysates (1:400):1-3. Anti-Oxytocin Receptor Antibody (#AVR-013).
4-6. Anti-Oxytocin Receptor Antibody preincubated with Oxytocin Receptor Blocking Peptide (#BLP-VR013).
Expression of Oxytocyn Receptor in rat midbrain.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Oxytocin Receptor Antibody (#AVR-013), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Oxytocyn Receptor immunoreactivity (green) appears in neuronal profiles (arrows) in the substantia nigra pars compacta (SNC). B. Pre-incubation of the antibody with Oxytocin Receptor Blocking Peptide (#BLP-VR013), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- CSARYLKGSRPGE, corresponding to amino acid residues 346-358 of rat Oxtr (Accession P70536).
Accession (Uniprot) Number P70536
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 3 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
