Overview
Type: Synthetic peptide
Form: Lyophilized powder
P2X1 Receptor (extracellular) Blocking Peptide (#BLP-PR022) is the original antigen used for immunization during Anti-P2X1 Receptor (extracellular) Antibody (#APR-022) generation. The blocking peptide binds and ‘blocks’ Anti-P2X1 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:1,2. Anti-P2X1 Receptor (extracellular) Antibody (#APR-022), (1:200).
3,4. Anti-P2X1 Receptor (extracellular) Antibody, preincubated with P2X1 Receptor (extracellular) Blocking Peptide (#BLP-PR022).
Expression of P2X1 Receptor in mouse cerebellumImmunohistochemical staining of mouse cerebellum using Anti-P2X1 Receptor (extracellular) Antibody (#APR-022). A. Most of P2RX1 labeling (green) appears in fine processes in the molecular layer (MOL) and in Purkinje cells (arrows show examples). B. DAPI is used as the counterstain (blue). C. Merge of A and B.
Properties
Sequence
- CRPIYEFHGLYEEK, corresponding to amino acid residues 270-283 of human P2X1 receptor (Accession P51575).
Accession (Uniprot) Number P51575
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
