Overview
Type: Synthetic peptide
Form: Lyophilized powder
P2X1 Receptor Blocking Peptide (#BLP-PR001) is the original antigen used for immunization during Anti-P2X1 Receptor Antibody (#APR-001) generation. The blocking peptide binds and ‘blocks’ Anti-P2X1 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human platelet lysates:1. Anti-P2X1 Receptor Antibody (#APR-001), (1:200).
2. Anti-P2X1 Receptor Antibody, preincubated with P2X1 Receptor Blocking Peptide (#BLP-PR001).
Expression of P2X1 Receptor in rat hypothalamusImmunohistochemical staining of rat supra-optic nucleus using Anti-P2X1 Receptor Antibody (#APR-001). P2X1 receptor (green) appears in axonal processes (vertical arrows) and cell bodies (horizontal arrows). DAPI is used as the counterstain (blue).- Mouse mesenteric arteries (Vial, C. and Evans, R.J. (2002) Mol. Pharmacol. 62, 1438.).
Properties
Sequence
- (CS)DPVATSSTLGLQENMRTS, corresponding to amino acid residues 382-399 of rat P2X1 receptor (Accession P47824).
Accession (Uniprot) Number P47824
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
