Overview
Type: Synthetic peptide
Form: Lyophilized powder
P2X2 Receptor (extracellular) Blocking Peptide (#BLP-PR025) is the original antigen used for immunization during Anti-P2X2 Receptor (extracellular) Antibody (#APR-025) generation. The blocking peptide binds and ‘blocks’ Anti-P2X2 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membrane (lanes 1 and 4), mouse brain membrane (lanes 2 and 5) and rat pheochromocytoma PC12 cells (lanes 3 and 6):1-3. Anti-P2X2 Receptor (extracellular) Antibody (#APR-025), (1:200).
4-6. Anti-P2X2 Receptor (extracellular) Antibody, preincubated with P2X2 Receptor (extracellular) Blocking Peptide (#BLP-PR025).
Properties
Sequence
- (C)KSDYLKHSTFDQDS, corresponding to amino acid residues 207-220 of rat P2X2 with replacement of cysteine 214 (C214) with serine (*S) (Accession P49653).
Accession (Uniprot) Number P49653
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
