Overview
Type: Synthetic peptide
Form: Lyophilized powder
P2X2 Receptor Blocking Peptide (#BLP-PR003) is the original antigen used for immunization during Anti-P2X2 Receptor Antibody (#APR-003) generation. The blocking peptide binds and ‘blocks’ Anti-P2X2 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of differentiated (lanes 1 and 3) and non-differentiated (lanes 2 and 4) PC12 cell lysates:1,2. Anti-P2X2 Receptor Antibody (#APR-003), (1:200).
3,4. Anti-P2X2 Receptor Antibody, preincubated with P2X2 Receptor Blocking Peptide (#BLP-PR003).
Expression of P2X2 receptor in rat neocortexImmunohistochemical staining of rat neocortex with Anti-P2X2 Receptor Antibody (#APR-003). P2X2 receptor (green) appears in axonal processes (arrows) that ascend toward the upper cortical layers (asterisk). DAPI is used as the counterstain (blue).
Expression of P2X2 receptor in rat hippocampusImmunohistochemical staining of rat hippocampus with Anti-P2X2 Receptor Antibody (#APR-003). P2X2 receptor (green) appears in the apical dendrites (arrows) that extend into the striatum radiatum (asterisk). DAPI is used as the counterstain (blue).- Mouse ilieum (1:200) (Ren, J. et al. (2003) J. Physiol. 552, 809.).
Properties
Sequence
- (C)SQQDSTSTDPKGLAQL, corresponding to amino acid residues 457-472 of rat P2X2 receptor (Accession P49653).
Accession (Uniprot) Number P49653
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
