Overview
Type: Synthetic peptide
Form: Lyophilized powder
P2X3 Receptor Blocking Peptide (#BLP-PR016) is the original antigen used for immunization during Anti-P2X3 Receptor Antibody (#APR-016) generation. The blocking peptide binds and ‘blocks’ Anti-P2X3 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat DRG lysates:1. Anti-P2X3 Receptor Antibody (APR-016), (1:200).
2. Anti-P2X3 Receptor Antibody, preincubated with P2X3 Receptor Blocking Peptide (#BLP-PR016).
Expression of P2X3 Receptor in rat DRG neuronsImmunohistochemical staining of rat dorsal root ganglion (DRG) neurons with Anti-P2X3 Receptor Antibody (#APR-016), (A). Cells within the DRG were stained (see solid line frame enlarged (B) as well as fibers and the area of entry of dorsal root into spinal cord (see dashed line frame enlarged (C). DAPI is used as the counterstain (in B and C).
Western blot analysis of rat dorsal root ganglion lysates:1. Guinea Pig Anti-P2X3 Antibody (#APR-016-GP), (1:400).
2. Guinea Pig Anti-P2X3 Antibody, preincubated with P2X3 Blocking Peptide (BLP-PR016).
Expression of P2X3 in rat dorsal root ganglion.Immunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglion (DRG) using Guinea pig Anti-P2X3 Antibody (#APR-016-GP), (1:200), followed by goat anti-guinea pig-AlexaFluor-594. A. P2X3 immunoreactivity (red) appears in neuronal profiles (arrows). B. Pre-incubation of the antibody with P2X3 Blocking Peptide (BLP-PR016), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of P2X3 in mouse paraventricular nucleus of the hypothalamus (PVN).Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Guinea pig Anti-P2X3 Antibody (#APR-016-GP), (1:200), followed by goat anti-guinea pig-AlexaFluor-594. A. P2X3 immunoreactivity (red) appears in neuronal profiles (arrows). B. Pre-incubation of the antibody with P2X3 Blocking Peptide (BLP-PR016), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = 3rd cerebral ventricle.
Properties
Sequence
- (C)VEKQSTDSGAYSIGH, corresponding to amino acid residues 383-397 of rat P2X3 receptor (Accession P49654).
Accession (Uniprot) Number P49654
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
