Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)KKYKYVEDYEQGLSGEMNQ, corresponding to amino acid residues 370-388 of rat P2X4 receptor (Accession P51577).
Accession (Uniprot) Number P51577
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-P2X4 Receptor Antibody (#APR-002), (1:200).
2. Anti-P2X4 Receptor Antibody, preincubated with P2X4 Receptor Blocking Peptide (#BLP-PR002).
Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and human U-87 MG glioblastoma cell (lanes 3 and 6) lysates:1,2. Guinea pig Anti-P2X4 Receptor Antibody (#APR-002-GP), (1:500).
3. Guinea pig Anti-P2X4 Receptor Antibody (1:1000).
4-6. Guinea pig Anti-P2X4 Receptor Antibody, preincubated with P2X4 Receptor Blocking Peptide (#BLP-PR002).
Last update: 02/01/2024
