Overview
Type: Synthetic peptide
Form: Lyophilized powder
P2X5 Receptor Blocking Peptide (#BLP-PR005) is the original antigen used for immunization during Anti-P2X5 Receptor Antibody (#APR-005) generation. The blocking peptide binds and ‘blocks’ Anti-P2X5 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-P2X5 Receptor Antibody (#APR-005), (1:200).
2. Anti-P2X5 Receptor Antibody, preincubated with P2X5 Receptor Blocking Peptide (#BLP-PR005).
Expression of P2RX5 in rat spinal cordImmunohistochemical staining of rat spinal cord frozen section using Anti-P2X5 Receptor Antibody (#APR-005), (1:100). P2RX5 staining (red) is specific for neurons in the anterior horn of the spinal cord. Hoechst 33342 is used as the counterstain.
Properties
Sequence
- (C)RENAIVNVKQSQILHPVKT, corresponding to amino acid residues 437-455 of rat P2X5 receptor (Accession P51578).
Accession (Uniprot) Number P51578
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
