Overview
Type: Synthetic peptide
Form: Lyophilized powder
P2X6 Receptor Blocking Peptide (#BLP-PR013) is the original antigen used for immunization during Anti-P2X6 Receptor Antibody (#APR-013) generation. The blocking peptide binds and ‘blocks’ Anti-P2X6 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-P2X6 Receptor Antibody (#APR-013), (1:200).
2. Anti-P2X6 Receptor Antibody, preincubated with P2X6 Receptor Blocking Peptide (#BLP-PR013).- Rat lens (1:200) (Suzuki Kerr, H. et al. (2009) Histochem. Cell. Biol. 131, 667.).
Expression of P2X6 in rat hypothalamus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections Anti-P2X6 Receptor Antibody (#APR-013), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. P2X6 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with P2X6 Blocking Peptide (BLP-PR013), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = Third ventricle.
Expression of P2X6 in rat parietal cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections Anti-P2X6 Receptor Antibody (#APR-013), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. P2X6 immunoreactivity (green) appears in cortical neurons (arrows). B. Pre-incubation of the antibody with P2X6 Blocking Peptide (BLP-PR013), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RTKYEEARAPKATTNSA, corresponding to amino acid residues 363-379 of rat P2X6 receptor (Accession P51579).
Accession (Uniprot) Number P51579
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
