Overview
Type: Synthetic peptide
Form: Lyophilized powder
P2X7 Receptor (extracellular) Blocking Peptide (#BLP-PR008) is the original antigen used for immunization during Anti-P2X7 Receptor (extracellular) Antibody (#APR-008) generation. The blocking peptide binds and ‘blocks’ Anti-P2X7 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 5) and human K562 chronic myelogenous leukemia cell line (lanes 2 and 6) and Mouse WEHI-231 B cell lymphoma (lanes 3 and 7) and human HL-60 promyelocytic leukemia cell line (lanes 4 and 8):1-4. Anti-P2X7 Receptor (extracellular) Antibody (#APR-008), (1:200).
5-8. Anti-P2X7 Receptor (extracellular) Antibody, preincubated with P2X7 Receptor (extracellular) Blocking Peptide (#BLP-PR008).
Expression of P2RX7 in rat pancreasImmunohistochemical staining of rat pancreas frozen section using Anti-P2X7 Receptor (extracellular) Antibody (#APR-008), (1:100), followed by mouse anti-rabbit-AlexaFluor-594 secondary antibody (red). Staining is present in endocrine cells of the Isle of Langerhans. Hoechst 33342 is used as the counterstain.
Properties
Sequence
- KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 receptor (Accession Q9H244).
Accession (Uniprot) Number Q9H244
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
