Overview
Type: Synthetic peptide
Form: Lyophilized powder
P2X7 Receptor Blocking Peptide (#BLP-PR004) is the original antigen used for immunization during Anti-P2X7 Receptor Antibody (#APR-004) generation. The blocking peptide binds and ‘blocks’ Anti-P2X7 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat hippocampus lysates:1. Anti-P2X7 Receptor Antibody (#APR-004), (1:200).
2. Anti-P2X7 Receptor Antibody, preincubated with P2X7 Receptor Blocking Peptide (#BLP-PR004).
Expression of P2X7 Receptor in rat pancreasImmunohistochemical staining of rat paraffin-embedded endocrine and exocrine pancreas sections using Anti-P2X7 Receptor Antibody (#APR-004), (1:50), followed by goat anti-rabbit-AlexaFluor-555-secondary antibody (1:500), (red). Staining is highly specific for endocrine cells of the Isle of Langerhans. Hoechst 33342 is used as the counterstain (blue).
Properties
Sequence
- (C)KIRKEFPKTQGQYSGFKYPY, corresponding to amino acid residues 576-595 of rat P2X7 receptor (Accession Q64663).
Accession (Uniprot) Number Q64663
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
