Overview
Type: Synthetic peptide
Form: Lyophilized powder
P2Y1 Receptor (extracellular) Blocking Peptide (#BLP-PR021) is the original antigen used for immunization during Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021) generation. The blocking peptide binds and ‘blocks’ Anti-P2Y1 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 4), Jurkat (lanes 2 and 5) and K-562 (lanes 3 and 6) lysates:1-3. Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021), (1:200).
4-6. Anti-P2Y1 Receptor (extracellular) Antibody, preincubated with P2Y1 Receptor (extracellular) Blocking Peptide (#BLP-PR021).
Expression of P2RY1 in mouse hippocampusImmunohistochemical staining of mouse hippocampal CA3 region using Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021). A. P2RY1 staining (red) appears in the pyramidal layer (arrows). B. Nuclei staining using DAPI as the counterstain (blue). C. Merged images of panels A and B.
Properties
Sequence
- SDEYLRSYFIYSMC, corresponding to amino acid residues 207-220 of human P2RY1 (Accession P47900).
Accession (Uniprot) Number P47900
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
