Overview
Type: Synthetic peptide
Form: Lyophilized powder
P2Y1 Receptor Blocking Peptide (#BLP-PR009) is the original antigen used for immunization during Anti-P2Y1 Receptor Antibody (#APR-009) generation. The blocking peptide binds and ‘blocks’ Anti-P2Y1 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and human platelets lysate (lanes 2 and 4):1,2. Anti-P2Y1 Receptor Antibody (#APR-009), (1:200).
3,4. Anti-P2Y1 Receptor Antibody, preincubated with P2Y1 Receptor Blocking Peptide (#BLP-PR009).
Expression of P2RY1 in rat thalamusImmunohistochemical staining of fasciculi in rat thalamus using Anti-P2Y1 Receptor Antibody (#APR-009). Specific staining is black. Cresyl violet is used as the counterstain.- Human eye tissue (Fries, J.E. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46, 3000.).
Expression of P2RY1 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-P2Y1 Receptor Antibody (#APR-009), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal dentate gyrus region, showed immunoreactivity (green) in in the sub-granular layer (arrows) and diffusely in the outer molecular layer (OML). B. Pre-incubation of the antibody with P2Y1 Receptor Blocking Peptide (#BLP-PR009), suppressed staining. Cell nuclei are stained with DAPI (blue). G = granule layer, OML = outer molecular layer.
Expression of P2RY1 in rat spinal cord.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-P2Y1 Receptor Antibody (#APR-009), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. P2Y1 immunoreactivity (green) appears in layers 1-2 of the dorsal horn (arrows). B. Pre-incubation of the antibody with P2Y1 Receptor Blocking Peptide (#BLP-PR009), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RALI YKDLD NSPLR RKS, corresponding to residues 242-258 of rat or human P2RY1. (Accession P49651).
Accession (Uniprot) Number P49651
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
