Overview
Type: Synthetic peptide
Form: Lyophilized powder
P2Y12 Receptor (extracellular) Blocking Peptide (#BLP-PR020) is the original antigen used for immunization during Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020) generation. The blocking peptide binds and ‘blocks’ Anti-P2Y12 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membrane (lanes 1 and 3) and human MEG-01 chronic myelogenous leukemia (lanes 2 and 4) cell line lysate:1-2. Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020), (1:200).
3-4. Anti-P2Y12 Receptor (extracellular) Antibody, preincubated with P2Y12 Receptor (extracellular) Blocking Peptide (#BLP-PR020).
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1,2. Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020-GP), (1:400).
3,4. Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody, preincubated with P2Y12 Receptor (extracellular) Blocking Peptide (#BLP-PR020).
Western blot analysis of rat basophilic leukemia (RBL) cell line lysate (lanes 5 and 7) and human brain glioblastoma (U-87 MG) cell line lysate (lanes 6 and 8):5,6. Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020-GP), (1:400).
7,8. Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody, preincubated with P2Y12 Receptor (extracellular) Blocking Peptide (#BLP-PR020).
Expression of P2Y12 in rat parietal cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020-GP), (1:300), followed by goat anti-guinea pig-AlexaFluor-594. A. P2Y12 immunoreactivity (red) appears in axonal processes (arrows). B. Pre-incubation of the antibody with P2Y12 Receptor (extracellular) Blocking Peptide (BLP-PR020), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- CTAENTLFYVKES, corresponding to amino acid residues 270-282 of human P2RY12 (Accession Q9H244).
Accession (Uniprot) Number Q9H244
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
