Overview
Type: Synthetic peptide
Form: Lyophilized Powder
P2Y13 Receptor (extracellular) Blocking Peptide (#BLP-PR103) is the original antigen used for immunization during Anti-P2Y13 Receptor (extracellular) Antibody (#APR-103) generation. The blocking peptide binds and ‘blocks’ Anti-P2Y13 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), rat dorsal root ganglia lysate (lanes 3 and 7) and rat spleen lysate (lanes 4 and 8):1-4. Anti-P2Y13 Receptor (extracellular) Antibody (#APR-103), (1:200).
5-8. Anti-P2Y13 Receptor (extracellular) Antibody, preincubated with P2Y13 Receptor (extracellular) Blocking Peptide (BLP-PR103).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 5), mouse BV-2 microglia cell line lysate (lanes 2 and 6), mouse J774 monocyte cell line lysate (lanes 3 and 7) and human HMC3 microglia cell line lysate (lanes 4 and 8):1-4. Anti-P2Y13 Receptor (extracellular) Antibody (#APR-103), (1:200).
5-8. Anti-P2Y13 Receptor (extracellular) Antibody, preincubated with P2Y13 Receptor (extracellular) Blocking Peptide (BLP-PR103).
Properties
Sequence
- Peptide (C)NKTD(S)RLENQLFIAKE, corresponding to amino acid residues 266 - 281 of mouse P2Y13 (Accession Q9D8I2).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
