Overview
Type: Synthetic peptide
Form: Lyophilized powder
P2Y14 Receptor (extracellular) Blocking Peptide (#BLP-PR018) is the original antigen used for immunization during Anti-P2Y14 Receptor (extracellular) Antibody (#APR-018) generation. The blocking peptide binds and ‘blocks’ Anti-P2Y14 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat heart membranes (lanes 1 and 4), brain (lanes 2 and 5) and lung lysates (lanes 3 and 6):1-3. Anti-P2Y14 Receptor (extracellular) Antibody (#APR-018), (1:200).
4-6. Anti-P2Y14 Receptor (extracellular) Antibody, preincubated with P2Y14 Receptor (extracellular) Blocking Peptide (#BLP-PR018).- Mouse kidney sections (Azroyan, A. et al. (2015) PLoS ONE 10, e0121419.).
Expression of P2Y14 in rat pancreas.Immunohistochemical staining of paraffin-embedded sections of rat pancreas with Anti-P2Y14 (extracellular) Antibody (#APR-018), (1:50), followed by SuperPicture HRP polymer. A. Staining in the rat pancreas showed P2Y14 immunoreactivity (brown) in endocrine cells of the Isles of Langerhans (arrows). Counterstain is Hematoxilin.
Properties
Sequence
- CIELKSELGRKWHKASN, corresponding to amino acid residues 172-188 of human P2RY14 (Accession Q15391).
Accession (Uniprot) Number Q15391
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
