Overview
Type: Synthetic peptide
Form: Lyophilized powder
P2Y6 Receptor (extracellular) Blocking Peptide (#BLP-PR106) is the original antigen used for immunization during Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106) generation. The blocking peptide binds and ‘blocks’ Anti-P2Y6 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) heart membranes:1,2. Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106), (1:400).
3,4. Anti-P2Y6 Receptor (extracellular) Antibody, preincubated with P2Y6 Receptor (extracellular) Blocking Peptide (#BLP-PR106).
Western blot analysis of mouse BV-2 microglia cell line lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1,2. Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106), (1:200).
3,4. Anti-P2Y6 Receptor (extracellular) Antibody, preincubated with P2Y6 Receptor (extracellular) Blocking Peptide (#BLP-PR106).
Western blot analysis of human MDA-MB-231 breast adenocarcinoma cell line lysate:1. Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106), (1:200).
2. Anti-P2Y6 Receptor (extracellular) Antibody, preincubated with P2Y6 Receptor (extracellular) Blocking Peptide (#BLP-PR106).
Expression of P2RY6 in rat parietal cortexImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-P2Y6 Receptor (extracellular) Antibody (#APR-106), (1:1000), followed by donkey-anti-rabbit-biotin and streptavidin-Cy3. P2RY6 immunoreactivity (red) appears in pyramidal neurons (arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RNRTV(S)YDLSPPILS, corresponding to amino acid residues 172 - 186 of mouse P2RY6 (Accession Q9ERK9).
Accession (Uniprot) Number Q9ERK9
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
