Overview
Type: Synthetic peptide
Form: Lyophilized powder
P2Y6 Receptor Blocking Peptide (#BLP-PR011) is the original antigen used for immunization during Anti-P2Y6 Receptor Antibody (#APR-011) generation. The blocking peptide binds and ‘blocks’ Anti-P2Y6 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat heart membranes:1. Anti-P2Y6 Receptor Antibody (#APR-011), (1:200).
2. Anti-P2Y6 Receptor Antibody, preincubated with P2Y6 Receptor Blocking Peptide (#BLP-PR011).- Mouse paraffin heart section.
- Human eye tissue (Fries, J.E. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46, 3000.).
Expression of P2Y6 in rat cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-P2Y6 Receptor Antibody (#APR-011), (1:600), followed by donkey anti-rabbit-biotin and Streptavidin-Cy3. A. P2Y6 immunoreactivity (red) appears in cortical neurons (horizontal arrows) and astrocytes (vertical arrows). B. Pre-incubation of the antibody with P2Y6 Receptor Blocking Peptide (BLP-PR011), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- CQPHDLLQKLTAKWQRQRV, corresponding to amino acid residues 311-328 of rat P2Y6 receptor (Accession Q63371).
Accession (Uniprot) Number Q63371
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
