Overview
Type: Synthetic peptide
Form: Lyophilized powder
p75 NGF Receptor Blocking Peptide (#BLP-NT011) is the original antigen used for immunization during Anti-p75 NGF Receptor (intracellular) Antibody (#ANT-011) generation. The blocking peptide binds and ‘blocks’ Anti-p75 NGF Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-p75 NGF Receptor (intracellular) Antibody (#ANT-011), (1:200).
2. Anti-p75 NGF Receptor (intracellular) Antibody, preincubated with p75 NGF Receptor Blocking Peptide (#BLP-NT011).
Western blot analysis of human melanoma cells A875:1. Anti-p75 NGF Receptor (intracellular) Antibody (#ANT-011), (1:200).
2. Anti-p75 NGF Receptor (intracellular) Antibody, preincubated with p75 NGF Receptor Blocking Peptide (#BLP-NT011).
Expression of p75NTR in mouse hippocampusImmunohistochemical staining of mouse frozen hippocampal sections using Anti-p75 NGF Receptor (intracellular) Antibody (#ANT-011) (1:200). A. p75NTR (green) appears in a subset of astrocytes. GFAP (glial fibrillary acidic protein) staining (red) to label astrocytes. C. A merge of the two images shows p75NTR found mainly in stratum radiatum (SOR) and only in a few astrocytes in the alveus (AL).
Properties
Sequence
- CKQNKQGANSRPVNQT corresponding to amino acid residues 278-293 of human p75NTR (Accession P08138).
Accession (Uniprot) Number P08138
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
