Overview
Type: Synthetic peptide
Form: Lyophilized Powder
PACC1/TMEM206 (extracellular) Blocking Peptide (#BLP-CL031) is the original antigen used for immunization during Anti-PACC1/TMEM206 (extracellular) Antibody (#ACL-031) generation. The blocking peptide binds and ‘blocks’ Anti-PACC1/TMEM206 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and rat kidney lysates (lanes 3 and 6):1-3. Anti-PACC1/TMEM206 (extracellular) Antibody (#ACL-031), (1:200).
4-6. Anti-PACC1/TMEM206 (extracellular) Antibody, preincubated with PACC1/TMEM206 (extracellular) Blocking Peptide (BLP-CL031).
Western blot analysis of human U-87 MG glioblastoma cell line lysate (lanes 1 and 5), human HT-29 colon adenocarcinoma cell line lysate (lanes 2 and 6), human THP-1 monocytic leukemia cell line lysate (lanes 3 and 7) and mouse BV-2 microglia cell line lysate (lanes 4 and 8):1-4. Anti-PACC1/TMEM206 (extracellular) Antibody (#ACL-031), (1:200).
5-8. Anti-PACC1/TMEM206 (extracellular) Antibody, preincubated with PACC1/TMEM206 (extracellular) Blocking Peptide (BLP-CL031).
Expression of PACC1 in mouse reticular thalamic nucleus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-PACC1/TMEM206 (extracellular) Antibody (#ACL-031), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. PACC1 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with PACC1/TMEM206 (extracellular) Blocking Peptide (BLP-CL031), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide ((C)KLKHPVMSVSYKEVDR, corresponding to amino acid residues 95 - 110 of mouse PACC1 (Accession Q9D771).
Accession (Uniprot) Number Q9D771
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
