Overview
Type: Synthetic peptide
Form: Lyophilized powder
PACSIN3 Blocking Peptide (#BLP-IP015) is the original antigen used for immunization during Anti-PACSIN3 Antibody (#AIP-015) generation. The blocking peptide binds and ‘blocks’ Anti-PACSIN3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membrane (lanes 1 and 5), rat skeletal muscle lysate (lanes 2 and 6), rat heart lysate (lanes 3 and 7) and mouse heart membrane (lanes 4 and 8):1-4. Anti-PACSIN3 Antibody (#AIP-015), (1:400).
5-8. Anti-PACSIN3 Antibody, preincubated with PACSIN3 Blocking Peptide (#BLP-IP015).
Properties
Sequence
- (C)RKDEKTAQTRESHAK, corresponding to amino acid residues 161-175 of mouse Pacsin3 (Accession Q99JB8).
Accession (Uniprot) Number Q99JB8
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
