Overview
Type: Synthetic peptide
Form: Lyophilized powder
Pan CaV α 1 Blocking Peptide (#BLP-CC004) is the original antigen used for immunization during Anti-CaVpan α1 Antibody (#ACC-004) generation. The blocking peptide binds and ‘blocks’ Anti-Pan CaV α 1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-CaVpan α1 Antibody (#ACC-004), (1:200).
2. Anti-CaVpan α1 Antibody, preincubated with Pan CaV α 1 Blocking Peptide (#BLP-CC004).- Human placenta (1:300) (Bernucci, L. et al. (2006) Placenta 27, 1082.).
Properties
Sequence
- (C)DNFDYLTRDWSILGPHHLD, corresponding to amino acid residues 1506-1524 of rat CaV1.2 (Accession P22002).
Accession (Uniprot) Number P22002
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
