Overview
Type: Synthetic peptide
Form: Lyophilized powder
Pan VDAC Blocking Peptide (#BLP-VC001) is the original antigen used for immunization during Anti-VDAC Antibody (#AVC-001) generation. The blocking peptide binds and ‘blocks’ Anti-Pan VDAC primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney (lanes 1 and 4), rat brain (lanes 2 and 5), mouse brain (lanes 3 and 6) membranes and human CCF-SSTGI brain astrocytoma (lanes 7 and 8) cell line lysate:1-3,7. Anti-VDAC Antibody (#AVC-001), (1:200).
4-6,8. Anti-VDAC Antibody, preincubated with Pan VDAC Blocking Peptide (#BLP-VC001).
Properties
Sequence
- (C)DGKNVNAGGHK, corresponding to amino acid residues of 264-274 of rat VDAC1 (Accession Q9Z2L0).
Accession (Uniprot) Number Q9Z2L0
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
