Overview
Type: Synthetic peptide
Form: Lyophilized powder
Pannexin 1 Blocking Peptide (#BLP-CC234) is the original antigen used for immunization during Anti-Pannexin 1 Antibody (#ACC-234) generation. The blocking peptide binds and ‘blocks’ Anti-Pannexin 1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 5) and rat (lanes 2 and 6) heart lysates and mouse (lanes 3 and 7) and rat (lanes 4 and 8) brain membranes:1-4. Anti-Pannexin 1 Antibody (#ACC-234), (1:400).
5-8. Anti-Pannexin 1 Antibody, preincubated with Pannexin 1 Blocking Peptide (#BLP-CC234).
Expression of Pannexin 1 in rat cortexImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Pannexin 1 Antibody (#ACC-234), (1:300). PANX1 staining (green) is apparent in cells with neuronal outline (vertical arrows) and apical dendrites (horizontal arrows) in the parietal cingulated cortex region. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KEPTEPKFKGLRLE, corresponding to amino acid residues 18- 31 of human PANX1 (Accession Q96RD7).
Accession (Uniprot) Number Q96RD7
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
