Overview
Type: Synthetic peptide
Form: Lyophilized powder
Pannexin 2 (extracellular) Blocking Peptide (#BLP-CC232) is the original antigen used for immunization during Anti-Pannexin 2 (extracellular) Antibody (#ACC-232) generation. The blocking peptide binds and ‘blocks’ Anti-Pannexin 2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-Pannexin 2 (extracellular) Antibody (#ACC-232), (1:200).
3,4. Anti-Pannexin 2 (extracellular) Antibody, preincubated with Pannexin 2 (extracellular) Blocking Peptide (#BLP-CC232).
Expression of Pannexin 2 in rat cerebellum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Pannexin 2 (extracellular) Antibody (#ACC-232), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Pannexin 2 immunoreactivity (green) appears in cells of the purkinje layer (horizontal arrows) and in segments of dendrites (vertical arrows) in the molecular layer (M). B. Pre-incubation of the antibody with Pannexin 2 (extracellular) Blocking Peptide (BLP-CC232), suppressed staining. Cell nuclei are stained with DAPI (blue). G = granule layer; M = molecular layer.
Expression of Pannexin 2 in mouse cerebellum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Pannexin 2 (extracellular) Antibody (#ACC-232), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Pannexin 2 immunoreactivity (green) appears in cells of the purkinje layer (horizontal arrows) and in segments of dendrites (vertical arrows) in the molecular layer (M). B. Pre-incubation of the antibody with Pannexin 2 (extracellular) Blocking Peptide (BLP-CC232), suppressed staining. Cell nuclei are stained with DAPI (blue). G = granule layer; M = molecular layer.
Properties
Sequence
- (C)EEPIY(S)YTPHNFTRD, corresponding to amino acid residues 76-90 of human PANX2 (Accession Q96RD6).
Accession (Uniprot) Number Q96RD6
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
