Overview
Type: Synthetic peptide
Form: Lyophilized powder
Pannexin 3 Blocking Peptide (#BLP-CC233) is the original antigen used for immunization during Anti-Pannexin 3 Antibody (#ACC-233) generation. The blocking peptide binds and ‘blocks’ Anti-Pannexin 3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) testis lysates:1,2. Anti-Pannexin 3 Antibody (#ACC-233), (1:200).
3,4. Anti-Pannexin 3 Antibody, preincubated with Pannexin 3 Blocking Peptide (#BLP-CC233).
Western blot analysis of rat skeletal muscle lysate:1. Anti-Pannexin 3 Antibody (#ACC-233), (1:200).
2. Anti-Pannexin 3 Antibody, preincubated with Pannexin 3 Blocking Peptide (#BLP-CC233).
Properties
Sequence
- (C)RLVQHMLKIRQKSSD, corresponding to amino acid residues 156-170 of human PANX3 (Accession Q96QZ0).
Accession (Uniprot) Number Q96QZ0
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
