Overview
Type: Synthetic peptide
Form: Lyophilized powder
PAR2/F2RL1 Blocking Peptide (#BLP-PR032) is the original antigen used for immunization during Anti-PAR2 (F2RL1) Antibody (#APR-032) generation. The blocking peptide binds and ‘blocks’ Anti-PAR2/F2RL1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human breast adenocarcinoma MDA-MB-231 (lanes 1 and 4) and MDA-MB-468 (lanes 2 and 5), and human lung small cell carcinoma NCI-H526 (lanes 3 and 6) cell lines:1-3. Anti-PAR2 (F2RL1) Antibody (#APR-032), (1:600).
4-6. Anti-PAR2 (F2RL1) Antibody, preincubated with PAR2/F2RL1 Blocking Peptide (#BLP-PR032).
Western blot analysis of rat lung (lanes 1 and 3) and liver (lanes 2 and 4) lysates:1,2. Anti-PAR2 (F2RL1) Antibody (#APR-032), (1:400).
3,4. Anti-PAR2 (F2RL1) Antibody, preincubated with PAR2/F2RL1 Blocking Peptide (#BLP-PR032).
Western blot analysis of mouse kidney lysates:1. Anti-PAR2 (F2RL1) Antibody (#APR-032), (1:400).
2. Anti-PAR2 (F2RL1) Antibody, preincubated with PAR2/F2RL1 Blocking Peptide (#BLP-PR032).
Western blot analysis of rat dorsal root ganglion lysates:1. Anti-PAR2 (F2RL1) Antibody (#APR-032), (1:200).
2. Anti-PAR2 (F2RL1) Antibody, preincubated with PAR2/F2RL1 Blocking Peptide (#BLP-PR032).- Human tendon sections (1:100) (Christensen, J. et al. (2015) Mol. Pain 11, 13.).
Properties
Sequence
- (C)KRMQISLTSNKFSRK, corresponding to amino acid residues 368-382 of rat PAR-2 (Accession Q63645).
Accession (Uniprot) Number Q63645
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
