Overview
Type: Synthetic peptide
Form: Lyophilized Powder
Parkin Blocking Peptide (#BLP-NR103) is the original antigen used for immunization during Anti-Parkin Antibody (#ANR-103) generation. The blocking peptide binds and ‘blocks’ Anti-Parkin primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain synaptosome preparation (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1-2. Anti-Parkin Antibody (#ANR-103), (1:200).
3-4. Anti-Parkin Antibody, preincubated with Parkin Blocking Peptide (BLP-NR103).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:1. Anti-Parkin Antibody (#ANR-103), (1:200).
2. Anti-Parkin Antibody, preincubated with Parkin Blocking Peptide (BLP-NR103).
Expression of Parkin in rat brain 4th ventricle.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Parkin Antibody (#ANR-103), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. Parkin immunoreactivity (green) appears along the wall of the 4th cerebral ventricle (arrows). B. Pre-incubation of the antibody with Parkin Blocking Peptide (BLP-NR103), suppressed staining. Cell nuclei are stained with DAPI (blue). 4th V= 4th ventricle.
Properties
Sequence
- Peptide (C)KELHHFRILGEEQYTR, corresponding to amino acid residues 298 - 313 of mouse Parkin (Accession Q9WVS6).
Accession (Uniprot) Number Q9WVS6
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
