Overview
Type: Synthetic peptide
Form: Lyophilized Powder
PepT2/SLC15A2 (extracellular) Blocking Peptide (#BLP-PT002) is the original antigen used for immunization during Anti-PepT2/SLC15A2 (extracellular) Antibody (#APT-002) generation. The blocking peptide binds and ‘blocks’ Anti-PepT2/SLC15A2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Anti-PepT2/SLC15A2 (extracellular) Antibody (#APT-002), (1:500).
3-4. Anti-PepT2/SLC15A2 (extracellular) Antibody, preincubated with PepT2/SLC15A2 (extracellular) Blocking Peptide (BLP-PT002).
Western blot analysis of rat kidney lysate:1. Anti-PepT2/SLC15A2 (extracellular) Antibody (#APT-002), (1:200).
2. Anti-PepT2/SLC15A2 (extracellular) Antibody, preincubated with PepT2/SLC15A2 (extracellular) Blocking Peptide (BLP-PT002).
Western blot analysis of human MCF-7 breast adenocarcinoma cell line lysate (lanes 1 and 4), human THP-1 monocytic leukemia cell line lysate (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell line lysate (lanes 3 and 6):1-3. Anti-PepT2/SLC15A2 (extracellular) Antibody (#APT-002), (1:200).
4-6. Anti-PepT2/SLC15A2 (extracellular) Antibody, preincubated with PepT2/SLC15A2 (extracellular) Blocking Peptide (BLP-PT002).
Expression of PepT2/SLC15A2 in rat subfornical organ.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-PepT2/SLC15A2 (extracellular) Antibody (#APT-002), (1:100), followed by goat anti-rabbit-AlexaFluor-488. A. PepT2 immunoreactivity (green) appears in cells of the subfornical organ (arrows). B. Pre-incubation of the antibody with PepT2/SLC15A2 (extracellular) Blocking Peptide (BLP-PT002), suppressed staining. Cell nuclei are stained with DAPI (blue). FX = fornix.
Properties
Sequence
- Peptide (C)KDYGVSEYRTVQRGK, corresponding to amino acid residues 541 - 555 of mouse SLC15A2 (Accession Q9ES07).
Accession (Uniprot) Number Q9ES07
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
