Overview
Type: Synthetic peptide
Form: Lyophilized powder
Periaxin Blocking Peptide (#BLP-NR091) is the original antigen used for immunization during Anti-Periaxin Antibody (#ANR-091) generation. The blocking peptide binds and ‘blocks’ Anti-Periaxin primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat DRG (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:1,2. Anti-Periaxin Antibody (#ANR-091), (1:600).
3,4. Anti-Periaxin Antibody, preincubated with Periaxin Blocking Peptide (#BLP-NR091).
Expression of Periaxin in rat peripheral nerveImmunohistochemical staining of free floating frozen rat peripheral nerve sections using Anti-Periaxin Antibody (#ANR-091), (#1:600). A. PRX staining (green) appears in a peripheral nerve (arrow) connecting to the spinal dorsal horn (D horn). Note the absence of staining of spinal myelin column indicating the specificity of the antibody to peripheral myelin. B. A higher magnification of the insert from panel A. C. Segment of peripheral nerve contains PRX-positive fibers. (arrows). In all panels DAPI is used as the counterstain (blue).
Western blot analysis of rat DRG lysate:1. Guinea pig Anti-Periaxin Antibody (#ANR-091-GP), (1:200).
2. Guinea pig Anti-Periaxin Antibody, preincubated with Periaxin Blocking Peptide (#BLP-NR091).
Western blot analysis of mouse lung lysate:3. Guinea pig Anti-Periaxin Antibody (#ANR-091-GP), (1:600).
4. Guinea pig Anti-Periaxin Antibody, preincubated with Periaxin Blocking Peptide (#BLP-NR091).
Expression of Periaxin in rat spinal dorsal root entry zoneImmunohistochemical staining of rat spinal dorsal root entry zone frozen sections using Guinea pig Anti-Periaxin Antibody (#ANR-091-GP). Periaxin immunoreactivity (red) appears in the dorsal nerve root (arrows). Nuclei are stained using DAPI as the counterstain (blue).
Properties
Sequence
- (C)DVEFSFPKFSRLRR, corresponding to amino acid residues 155-170 of rat PRX (Accession Q63425).
Accession (Uniprot) Number Q63425
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
