Overview
Type: Synthetic peptide
Form: Lyophilized powder
PEX5L/TRIP8b Blocking Peptide (#BLP-PR070) is the original antigen used for immunization during Anti-PEX5L (TRIP8b) Antibody (#APR-070) generation. The blocking peptide binds and ‘blocks’ Anti-PEX5L/TRIP8b primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-PEX5L (TRIP8b) Antibody (#APR-070), (1:400).
3,4. Anti-PEX5L (TRIP8b) Antibody, preincubated with PEX5L/TRIP8b Blocking Peptide (#BLP-PR070).
Expression of PEX5-related protein in rat brainImmunohistochemical staining of rat parietal cortex using Anti-PEX5L (TRIP8b) Antibody (#APR-070), (1:300). A. PEX5Rp (green) is labeled in dendritic and axonal profiles (arrows). B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.
Properties
Sequence
- (C)EKWDDVKFHGDRTSK, corresponding to amino acid residues 151-165 of rat PEX5-related protein (Accession Q925N3).
Accession (Uniprot) Number Q925N3
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
