Cat #: AK-465_KIT
Size: 18 Vials
Form: Lyophilized
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Anti-KCNK1 (TWIK-1) (extracellular) Antibody Cat #: APC-110
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide RQELRKLKRRFLEEHEC, corresponding to amino acid residues 53-69 of human KCNK1 (Accession O00180). Extracellular, near the P1 loop.
Homology
Rat, mouse, pig - identical.
KCNK1/TWIK-1 (extracellular) Blocking Peptide Cat #: BLP-PC110
Size:
40 µg
Anti-KCNK2 (TREK-1) Antibody Cat #: APC-047
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide DPKSAAQNSKPRLSFSTK(C), corresponding to residues 8-25 of human KCNK2 (Accession O95069). Intracellular, N-terminus.
Homology
Rat - identical; mouse - 17/18 amino acid residues identical.
KCNK2/TREK-1 Blocking Peptide Cat #: BLP-PC047
Size:
40 µg
Anti-KCNK3 (TASK-1) Antibody Cat #: APC-024
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, R
Immunogen
Peptide (C)EDEKRDAEHRALLTRNGQ, corresponding to amino acid residues 252-269 of human KCNK3 (Accession O14649). Intracellular, C-terminal part.
Homology
Rat, mouse - 17/18 amino acid residues identical. Homology with human TASK-3: 11/17 amino acid residues identical.
KCNK3/TASK-1 Blocking Peptide Cat #: BLP-PC024
Size:
40 µg
Anti-KCNK4 (TRAAK) Antibody Cat #: APC-108
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide NLAFIDESSDTQSERGC, corresponding to amino acid residues 343-359 of human KCNK4 (Accession Q9NYG8). Intracellular, C-terminus.
Homology
Rat, mouse, dog - identical.
KCNK4/TRAAK Blocking Peptide Cat #: BLP-PC108
Size:
40 µg
Anti-KCNK5 (TASK-2) Antibody Cat #: APC-037
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)YEQLMNEYNKANSPKGT, amino acid residues 483-499 of human KCNK5 (Accession number O95279). Intracellular, C-terminus.
Homology
Mouse - 14/17 amino acid residues identical.
KCNK5/TASK-2 Blocking Peptide Cat #: BLP-PC037
Size:
40 µg
Anti-KCNK6 (TWIK-2) Antibody Cat #: APC-040
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
WB
Reactivity:
H, R
Immunogen
Peptide (C)ESHQQLSASSHTDYASIPR, corresponding to residues 295-313 of human KCNK6 (Accession Q9Y257). Intracellular, C-terminus.
Homology
Rat - 13/17 amino acid residues identical.
KCNK6/TWIK-2 Blocking Peptide Cat #: BLP-PC040
Size:
40 µg
Anti-KCNK9 (TASK-3) (extracellular) Antibody Cat #: APC-044
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, R
Immunogen
Peptide (C)DDYQQLELVILQSEPHR, corresponding to amino acid residues 57-73 of rat KCNK9 (accession number Q9ES08). Extracellular, near the P1 loop.
Homology
Human - 15/17 amino acid residues identical.
KCNK9/TASK-3 (extracellular) Blocking Peptide Cat #: BLP-PC044
Size:
40 µg
Anti-KCNK10 (TREK-2) Antibody Cat #: APC-055
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, R
Immunogen
Peptide TFRNYSLDEEKKEDETEKMC, corresponding to amino acid residues 475-494 of rat KCNK10 (Accession Q9JIS4). Intracellular, C-terminal domain.
Homology
Human - 19/20 amino acid residues identical.
KCNK10/TREK-2 Blocking Peptide Cat #: BLP-PC055
Size:
40 µg
Anti-KCNK16 (TALK-1) (extracellular) Antibody Cat #: APC-170
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)EAWVKGVNPKGNST, corresponding to amino acid residues 75-88 of human KCNK16 (Accession Q96T55). 1st extracellular loop.
Homology
Rat, mouse – identical.
KCNK16/TALK-1 (extracellular) Blocking Peptide Cat #: BLP-PC170
Size:
40 µg
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
For research purposes only, not for human use
