Overview
Type: Synthetic peptide
Form: Lyophilized powder
PICK1 Blocking Peptide (#BLP-PZ014) is the original antigen used for immunization during Anti-PICK1 Antibody (#APZ-014) generation. The blocking peptide binds and ‘blocks’ Anti-PICK1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human PANC-1 pancreatic carcinoma cell lysate (lanes 1 and 3) and human HepG2 liver carcinoma cell lysate (lanes 2 and 4):1,2. Anti-PICK1 Antibody (#APZ-014), (1:200).
3,4. Anti-PICK1 Antibody, preincubated with PICK1 Blocking Peptide (#BLP-PZ014).
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:1,2. Anti-PICK1 Antibody (#APZ-014), (1:200).
3,4. Anti-PICK1 Antibody, preincubated with PICK1 Blocking Peptide (#BLP-PZ014).
Expression of PICK1 in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-PICK1 Antibody (#APZ-014), (1:400), followed by anti-rabbit-Cy3 antibody. PICK1 staining (red in the hippocampal CA3 region) is detected in neurons (arrows). Nuclei are stained with DAPI (blue).
Multiplex staining of GluR1 and PICK1 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea pig Anti-GluR1 (GluA1) (extracellular) Antibody (#AGC-004-GP), (1:400), followed by goat anti-guinea pig-AlexaFluor-488 and Anti-PICK1 Antibody (#APZ-014), (1:400), followed by donkey anti-rabbit-Cy3. A. GluR1 immunoreactivity (green) appears in several interneurons (arrows). B. PICK1 immunoreactivity (red) appears also in interneurons (arrows). C. Merge of the two images reveals co-localization in several interneurons (arrows) in the dentate gyrus (DG). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RARFSQMRKDVLEK, corresponding to amino acid residues 305-318 of rat PICK1 (Accession Q9EP80).
Accession (Uniprot) Number Q9EP80
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
