Overview
Type: Synthetic peptide
Form: Lyophilized powder
Piezo1 Blocking Peptide (#BLP-PC087) is the original antigen used for immunization during Anti-Piezo1 Antibody (#APC-087) generation. The blocking peptide binds and ‘blocks’ Anti-Piezo1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat lung (lanes 1 and 3) and mouse lung (lanes 2 and 4) lysates:1,2. Anti-Piezo1 (extracellular) Antibody (#APC-087), (1:600).
3,4. Anti-Piezo1 Antibody, preincubated with Piezo1 Blocking Peptide (#BLP-PC087).
Expression of Piezo1 in rat olfactory bulbImmunohistochemical staining of rat olfactory bulb sections using Anti-Piezo1 (extracellular) Antibody (#APC-087). A. Piezo1 staining (green) appears in the neuropil with varying intensity within and between glomeruli. B. Nuclear staining (blue) using DAPI as the counterstain. C. Merged image of panels A and B.
Properties
Sequence
- (C)EDLKPQHRRHISIR, corresponding to amino acid residues 1863-1876 of rat Piezo1 (Accession Q0KL00).
Accession (Uniprot) Number Q0KL00
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
