Overview
Type: Synthetic peptide
Form: Lyophilized powder
Plexin-A1 (extracellular) Blocking Peptide (#BLP-PR081) is the original antigen used for immunization during Anti-Plexin-A1 (extracellular) Antibody (#APR-081) generation. The blocking peptide binds and ‘blocks’ Anti-Plexin-A1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain synaptosomal fractions:1,2. Anti-Plexin-A1 (extracellular) Antibody (#APR-081), (1:400).
3,4. Anti-Plexin-A1 (extracellular) Antibody, preincubated with Plexin-A1 (extracellular) Blocking Peptide (#BLP-PR081).
Multiplex staining of GluN1 and Plexin-A1 in rat olfactory bulbImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001-GP), (1:600) and rabbit Anti-Plexin-A1 (extracellular) Antibody (#APR-081), (1:400). A. GluN1 (green) is expressed in the glomeruli (horizontal arrows). B. PLXNA1 staining (red) in the same section, shows expression in the glomeruli (arrow) but also in other layers (vertical arrow) of the olfactory bulb. C. Merge of the two images showcases co-localization in the glomeruli vs. lack of co-localization in deeper layers of the olfactory bulb. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)REVAPITRGQGDQR, corresponding to amino acid residues 623-636 of human PLXNA1 (Accession Q9UIW2).
Accession (Uniprot) Number Q9UIW2
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
