Overview
Type: Synthetic peptide
Form: Lyophilized powder
Plexin-A2 (extracellular) Blocking Peptide (#BLP-PR082) is the original antigen used for immunization during Anti-Plexin-A2 (extracellular) Antibody (#APR-082) generation. The blocking peptide binds and ‘blocks’ Anti-Plexin-A2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-Plexin-A2 (extracellular) Antibody (#APR-082), (1:200).
3,4. Anti-Plexin-A2 (extracellular) Antibody, preincubated with Plexin-A2 (extracellular) Blocking Peptide (#BLP-PR082).
Multiplex staining of Plexin-A2 and p75NTR in rat brainImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Plexin-A2 (extracellular) Antibody (#APR-082), (1:400) and mouse Mouse Anti-Rat p75 NGF Receptor (extracellular) Antibody (#AN-170), (1:300). A. Sections of rat horizontal diagonal band (HDB) were stained for PLXNA2 (green). B. Same sections were stained for p75NTR (red). C. Merge of the two images reveals cells expressing both PLXNA2 and p75NTR (horizontal arrows), cells expressing only PLXNA2 (upwards pointing arrows) or cells expressing only p75NTR (downwards pointing arrows) can also be observed. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RDPESSAMLDYELH, corresponding to amino acid residues 205-218 of mouse PLXNA2 (Accession P70207).
Accession (Uniprot) Number P70207
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
