Overview
Type: Synthetic peptide
Form: Lyophilized powder
Plexin-A3 (extracellular) Blocking Peptide (#BLP-PR093) is the original antigen used for immunization during Anti-Plexin-A3 (extracellular) Antibody (#APR-093) generation. The blocking peptide binds and ‘blocks’ Anti-Plexin-A3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-Plexin-A3 (extracellular) Antibody (#APR-093), (1:200).
3,4. Anti-Plexin-A3 (extracellular) Antibody, preincubated with Plexin-A3 (extracellular) Blocking Peptide (#BLP-PR093).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate:1. Anti-Plexin-A3 (extracellular) Antibody (#APR-093), (1:200).
2. Anti-Plexin-A3 (extracellular) Antibody, preincubated with Plexin-A3 (extracellular) Blocking Peptide (#BLP-PR093).
Expression of Plexin-A3 in mouse parietal cortexImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Plexin-A3 (extracellular) Antibody (#APR-093), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. PLXNA3 staining (green) appears in pyramidal neurons (arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KVRVDGSQDAQLYE, corresponding to amino acid residues 440-453 of mouse PLXNA3 (Accession P70208).
Accession (Uniprot) Number P70208
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
