Overview
Type: Synthetic peptide
Form: Lyophilized Powder
Plexin-C1 (extracellular) Blocking Peptide (#BLP-PR091) is the original antigen used for immunization during Anti-Plexin-C1 (extracellular) Antibody (#APR-091) generation. The blocking peptide binds and ‘blocks’ Anti-Plexin-C1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 4), rat brain membranes (lanes 2 and 5) and rat new born brain membranes (lanes 3 and 6):1-3. Anti-Plexin-C1 (extracellular) Antibody (#APR-091), (1:200).
4-6. Anti-Plexin-C1 (extracellular) Antibody, preincubated with Plexin-C1 (extracellular) Blocking Peptide (BLP-PR091).
Western blot analysis of human HL-60 acute promyelocytic leukemia cell line lysates (lanes 1 and 3) and human Malme-3M melanoma cell line lysate (lanes 2 and 4):1-2. Anti-Plexin-C1 (extracellular) Antibody (#APR-091), (1:200).
3-4. Anti-Plexin-C1 (extracellular) Antibody, preincubated with Plexin-C1 (extracellular) Blocking Peptide (BLP-PR091).
Expression of Plexin-C1 in in rat dorsal root ganglion.Immunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglion (DRG) sections with Anti-Plexin-C1 (extracellular) Antibody (#APR-091), (1: 200), followed by goat anti-rabbit-AlexaFluor-488. A. Plexin-C1 immunoreactivity (green) appears in DRG neuron profiles (arrows). B. Pre-incubation of the antibody with Plexin-C1 (extracellular) Blocking Peptide (BLP-PR091), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)EVKIQKENDNFNISK, corresponding to amino acid residues 860 - 874 of human Plexin-C1 (Accession O60486).
Accession (Uniprot) Number O60486
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
