Overview
Type: Synthetic peptide
Form: Lyophilized Powder
PMAT (SLC29A4) Blocking Peptide (#BLP-NT054) is the original antigen used for immunization during Anti-PMAT (SLC29A4) Antibody (#ANT-054) generation. The blocking peptide binds and ‘blocks’ Anti-PMAT (SLC29A4) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysates (lanes 2 and 4):1-2. Anti-PMAT (SLC29A4) Antibody (#ANT-054), (1:400).
3-4. Anti-PMAT (SLC29A4) Antibody, preincubated with PMAT (SLC29A4) Blocking Peptide (BLP-NT054).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate (lanes 1 and 3) and human Caco2 colorectal adenocarcinoma cell line lysate (lanes 2 and 4):1-2. Anti-PMAT (SLC29A4) Antibody (#ANT-054), (1:400).
3-4. Anti-PMAT (SLC29A4) Antibody, preincubated with PMAT (SLC29A4) Blocking Peptide (BLP-NT054).
Expression of PMAT in rat supraoptic nucleus (SON).Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-PMAT (SLC29A4) Antibody (#ANT-054), (1:1000), followed by donkey anti-rabbit-biotin and Streptavidin-Cy3. A. PMAT immunoreactivity (red) appears in SON neurons (arrows). B. Pre-incubation of the antibody with PMAT (SLC29A4) Blocking Peptide (BLP-NT054), suppressed staining. Cell nuclei are stained with DAPI (blue). Abbreviations: OT = optic tract, SON = supraoptic nucleus.
Properties
Sequence
- Peptide (C)GVYMRFDVPRPRVKR, corresponding to amino acid residues 315 - 329 of mouse SLC29A4 (Accession Q8R139).
Accession (Uniprot) Number Q8R139
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
