Overview
Type: Synthetic peptide
Form: Lyophilized powder
PMCA1 Blocking Peptide (#BLP-CP005) is the original antigen used for immunization during Anti-PMCA1 Antibody (#ACP-005) generation. The blocking peptide binds and ‘blocks’ Anti-PMCA1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-PMCA1 Antibody (#ACP-005), (1:200).
3,4. Anti-PMCA1 Antibody, preincubated with PMCA1 Blocking Peptide (#BLP-CP005).
Expression of PMCA1 in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-PMCA1 Antibody (#ACP-005), (1:300), followed by goat anti-rabbit-AlexaFluor-488 antibody. PMCA1 staining (green) appears in the stratum radiatum (SR) and oriens (OR) layers but not in the pyramidal (Pyr) layer of hippocampal CA1 region (arrows). Nuclei are labeled with DAPI (blue).
Properties
Sequence
- (C)SGVKNSLKEANHD, corresponding to amino acid residues 12-24 of human PMCA1 (Accession P20020).
Accession (Uniprot) Number P20020
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
