Overview
Type: Synthetic peptide
Form: Lyophilized powder
Presenilin-1 Blocking Peptide (#BLP-IP011) is the original antigen used for immunization during Anti-Presenilin-1 Antibody (#AIP-011) generation. The blocking peptide binds and ‘blocks’ Anti-Presenilin-1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat pancreas membrane (lanes 1 and 4), rat hippocampus (lanes 2 and 5) and mouse pancreatic cancer cell line (MS1) (lanes 3 and 6):1-3. Anti-Presenilin-1 Antibody (#AIP-011), (1:500).
4-6. Anti-Presenilin-1 Antibody, preincubated with Presenilin-1 Blocking Peptide (#BLP-IP011).
Expression of Presenilin-1 in mouse brainImmunohistochemical staining of mouse hippocampus and mouse cerebellum using Anti-Presenilin-1 Antibody (#AIP-011), (1:600). A. In hippocampus, PS-1 staining (green) appears in apical dendrites of pyramidal neurons (arrows). B. In cerebellum, an intense staining for PS-1 (green) appears in dendritic trees of Purkinje cells (arrows). A moderate staining is detected in the granule layer and in the soma of Purkinje and molecular layer interneurons. In both panels DAPI is used as the counterstain.
Properties
Sequence
- (C)RDSHLGPHRSTPESR, corresponding to amino acid residues 345-359 of rat Psen1 (Accession P97887).
Accession (Uniprot) Number P97887
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
