Overview
Type: Synthetic peptide
Form: Lyophilized Powder
Progranulin Blocking Peptide (#BLP-NR178) is the original antigen used for immunization during Anti-Progranulin Antibody (#ANR-178) generation. The blocking peptide binds and ‘blocks’ Anti-Progranulin primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysates (lanes 1 and 3) and rat spleen membranes (lanes 2 and 4):1-2. Anti-Progranulin Antibody (#ANR-178), (1:400).
3-4. Anti-Progranulin Antibody, preincubated with Progranulin Blocking Peptide (BLP-NR178).
Western blot analysis of human U-87 MG glioblastoma cell line lysate (lanes 1 and 5), human K562 myelogenous leukemia cell line lysate (lanes 2 and 6), human MCF-7 breast adenocarcinoma cell line lysate (lanes 3 and 7) and mouse BV-2 microglia cell line lysate (lanes 4 and 8):1-4. Anti-Progranulin Antibody (#ANR-178), (1:400).
5-8. Anti-Progranulin Antibody, preincubated with Progranulin Blocking Peptide (BLP-NR178).
Expression of progranulin in rat hypothalamus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Progranulin Antibody (#ANR-178), (1:100), followed by goat anti-rabbit-AlexaFluor-488. A. Progranulin immunoreactivity (green) appears in neurons (arrows pointing to the left) and along the wall of 3rd ventricle (arrows pointing to the right). B. Pre-incubation of the antibody with Progranulin Blocking Peptide (BLP-NR178), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V= Third ventricle.
Properties
Sequence
- Peptide CDLIQSK(S)LSKNYTTD, corresponding to amino acid residues 252 - 267 of mouse GRN (Accession P28798).
Accession (Uniprot) Number P28798
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
