Overview
Type: Synthetic peptide
Form: Lyophilized powder
Prokineticin Receptor 1 (extracellular) Blocking Peptide (#BLP-PR041) is the original antigen used for immunization during Anti-Prokineticin Receptor 1 (extracellular) Antibody (#APR-041) generation. The blocking peptide binds and ‘blocks’ Anti-Prokineticin Receptor 1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-Prokineticin Receptor 1 (extracellular) Antibody (#APR-041), (1:200).
2. Anti-Prokineticin Receptor 1 (extracellular) Antibody, preincubated with Prokineticin Receptor 1 (extracellular) Blocking Peptide (#BLP-PR041).
Western blot analysis of mouse brain lysate:1. Anti-Prokineticin Receptor 1 (extracellular) Antibody (#APR-041), (1:200).
2. Anti-Prokineticin Receptor 1 (extracellular) Antibody, preincubated with Prokineticin Receptor 1 (extracellular) Blocking Peptide (#BLP-PR041).
Expression of Prokineticin receptor 1 in rat DRGImmunohistochemical staining of frozen rat dorsal root ganglion (DRG) sections using Anti-Prokineticin Receptor 1 (extracellular) Antibody (#APR-041), (1:100), followed by Alexa-555-conjugated goat anti-rabbit secondary antibody. PK-R1 (red) is expressed in DRG neurons. Cell nuclei are visualized with Hoechst 33342 (blue).
Expression of Prokineticin receptor 1 in mouse brainImmunohistochemical staining of mouse olfactory bulb brain sections using Anti-Prokineticin Receptor 1 (extracellular) Antibody (#APR-041), (1:100). A. Green fluorescence reveals intensely stained astrocyte-like cells in the glomeruli (G) and lightly stained astrocyte-like cells in the adjacent layer (arrows). B. Merge of images of PK-R1 (green), parvalbumin (red, a neuronal marker) and DAPI (blue) nuclear counterstain.
Properties
Sequence
- (C)ENTTNTFTDFFSARD, corresponding to amino acid residues 10-24 of rat PROKR1 (Accession Q8R416).
Accession (Uniprot) Number Q8R416
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
